How to generate shoots from a rooted leaf?

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gopat
Garter
Garter
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Joined: Fri Mar 25, 2005 4:19 am

How to generate shoots from a rooted leaf?

Post by gopat » Fri Mar 25, 2005 4:32 am

Hi,
I was able to generate roots to grow from the petiole of a Poplar leaf that I soaked in a nutrient solution for 10 days. The leaf and roots are very healthy. How can I generate shoots from this material?

I would like to use this method to create somaclonal variants and perhaps tetraploids.
Thanks,
Pat

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JackBean
Inland Taipan
Inland Taipan
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Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Sat Jun 19, 2010 5:57 pm

put it onto regenerating media. Add some cytokinins ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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