Yeast fermentative characteristics

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Nithin
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Yeast fermentative characteristics

Post by Nithin » Sun Mar 05, 2006 1:29 pm

Greetings,
I am Nithin doing final year Biotechnology and Genetic engineering. I am currently diong a project on fermentation. Can anyone please tell me how to improve yeast fermentative characteristics genetically?? Yeast acts on Mollases to produce ethanol and Carbod-di-oxide. I want to increase the production of ethanol.

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Post by fushxmy » Sun Mar 05, 2006 2:18 pm

Delete FPS1,GPD1,GPD1 .overexpress GLT1 or GLN1.

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Post by Nithin » Sun Mar 05, 2006 3:08 pm

Any idea how to do it!!!
I was also suggested to over express SUC2 and YPLO88W.
Anymore great ideas.
:?: :idea:

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Post by 12345 » Wed Mar 08, 2006 10:13 am

How to identify the various genes responsible for various activities. And after identifying them how to cause an overexpression of a particular gene. Please??

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Post by canalon » Wed Mar 08, 2006 12:48 pm

For the first step, microarrays, when available, are a good way to go. Otherwise a lot tedious analysis of expression level using things like RT-PCR and substractive hybridization for example.

Second step, clone it in a vector with a controled promoter is probably the simple answer. But if you want to do it in physiological conditions, I have no idea.
Patrick

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any proof. (Ashley Montague)

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