Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
Moderators: honeev, Leonid, amiradm, BioTeam
- Posts: 2
- Joined: Sun Feb 26, 2006 10:57 pm
Why can oligonucleotides be a limiting component of the PCR(polymerase chain reaction) reaction. Considering this what other component of the PCR reaction could also be considered rate limiting?
- King Cobra
- Posts: 586
- Joined: Sat Jul 30, 2005 7:16 pm
- Location: holland
imagine when you don't have enough dNTPs
other limiting components can be: Taq-polymerase/cofactor for polymerase / primers (forming dimers and thus inactivation)
Who is online
Users browsing this forum: No registered users and 7 guests