Polymerase chain reaction

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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intrigued
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Polymerase chain reaction

Post by intrigued » Sun Feb 26, 2006 11:03 pm

Why can oligonucleotides be a limiting component of the PCR(polymerase chain reaction) reaction. Considering this what other component of the PCR reaction could also be considered rate limiting?

sdekivit
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Post by sdekivit » Mon Feb 27, 2006 8:53 am

imagine when you don't have enough dNTPs ;) other limiting components can be: Taq-polymerase/cofactor for polymerase / primers (forming dimers and thus inactivation)

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