Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Why can oligonucleotides be a limiting component of the PCR(polymerase chain reaction) reaction. Considering this what other component of the PCR reaction could also be considered rate limiting?
- King Cobra
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imagine when you don't have enough dNTPs
other limiting components can be: Taq-polymerase/cofactor for polymerase / primers (forming dimers and thus inactivation)
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