Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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I had isolated PMogen22 binary vector about 11.5 kb size and wanted to digest it with Hind III and EcorI enzymw. This is supposed to be a very simple digestion but somehow my dna gets degraded, dont know why? Have tired using kit to isolate plasmid and other protocols also but all the digestions dosent work. Can some one suggest why this happens.
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