Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
Moderators: Leonid, amiradm, BioTeam
- Posts: 10
- Joined: Tue Feb 21, 2006 8:18 am
My genomic DNA is around 6.1Mb. After DNA extraction, how am I going to confirm that I have extract the DNA?
- King Cobra
- Posts: 586
- Joined: Sat Jul 30, 2005 7:16 pm
- Location: holland
you can measure absorbance of light at 280 nm and 260 nm The absorptionmaximum of DNA/RNA is at 260 nm and of protein at 280 nm
--> when pure DNA is isolated, then the ratio 260/280 will be approximately 1,8
Now you do know there is DNA (or not) and how pure it is.
- Posts: 364
- Joined: Sat Dec 31, 2005 12:45 am
- Location: Manchester
Another way is a restriction digest using restriction enzymes and running the samples afterwards through electrophoresis. I do that all the time at uni. Info on electrophoresis can be found in tounes of bio textbooks.
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