Thawing of competent bacterial cells for transformation

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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summer
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Thawing of competent bacterial cells for transformation

Post by summer » Mon Mar 21, 2005 2:52 am

How can I thaw competent bacterial cells for transformation? Can i thaw the tube within my hand or would this damage my cells?

zerotimbo2001
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Post by zerotimbo2001 » Tue Mar 22, 2005 4:13 pm

yes gentle thawing is required, as it reduces, mechanical damage to cell walls and hence reduce viable counts...... intracellular water crystal formation and abberations to the PDG are all factors which need to be considered..... when frezzing cells of any sort

make sure to use asceptic technique at all times

Oh yeah thaw and place on ice.

muntedkowhai
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Post by muntedkowhai » Thu May 19, 2005 8:31 am

actually you should thaw the competent bugs on ice, dont thaw and then put on ice.
it takes a while but this is better for the bugs.
imagine the shock of thawing it with your hands and then getting stuck back on ice.

sara135
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Re: Thawing of competent bacterial cells for transformation

Post by sara135 » Tue Jul 12, 2011 11:55 am

thawing the competent cells with hands will definitely harm them. its always better to thaw them by keeping them in ice.....

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