Catalase...

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Byrne
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Catalase...

Post by Byrne » Wed Feb 22, 2006 10:04 pm

Just wondering if anyone has some particular information on this enzyme as I'm having some trouble on the Internet.

I completed a lab today using the catalase enzyme, examing the factors that affect the biological catalyst as it speeds up the decomposition of hydogen peroxide. The information I am finding on the Internet about pH suggests that its optimal pH is around 7.0, but when we performed the lab, the reaction proceeded quickist in the 10-11 pH range. Also, regarding temperature, we had to heat the liver "milkshake" we had to a temperature of 75 C... according to information I've found, denaturation begins signifcantly below this mark, so should be see decreased activity from our room temp experimentation?

Coraleen212
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Post by Coraleen212 » Wed Mar 01, 2006 9:42 am

I would say that catalase would have denatured significantly before 75 C. I think its optimum temperature is around 35-40 C and would start to denature at around 45 C ish. So if it was exposed to temperatures of 75 C, then yes, I'd say you should expect a decreased rate. I'm not certain as to its optimum pH, however I think itis likely to be around pH 7, as extremes in pH would denature it as well.

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