A new protein molecule..

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ambercote
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A new protein molecule..

Post by ambercote » Wed Feb 22, 2006 12:01 am

What is the formula for the production of a new protein molecule??

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canalon
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Post by canalon » Wed Feb 22, 2006 12:16 am

Create the appropriate DNA sequence, insert in a selectable plasmid with a good promoter (plenty are available on the market) transform an innocent E. coli, select and feed and you have plenty of your new protein.
This is a rather rough sketch to answer a vague question.
Patrick

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any proof. (Ashley Montague)

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Post by blazerUAB » Wed Feb 22, 2006 4:20 am

Try and be a little a more specific. I think I know what you mean but clarify by telling me what you know

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canalon
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Post by canalon » Wed Feb 22, 2006 4:42 am

To be clearer what you need is a gene that will code for the protein, and a translation system. You can try acellular all the way (mix of synthetic RNA and ribosomes with the right buffers and molecules) or more simply put your bioengineered gene in a host as bacteria. You need to choose a vector adapted to your host (bacteria here, but could be eukaryotic cell). A way to select the cells that have your gene is usefull too :)
Patrick

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any proof. (Ashley Montague)

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Post by ambercote » Thu Feb 23, 2006 5:17 am

Alright thanks guys.

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