PCR of long genomic DNA (bacteria)

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daniell
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PCR of long genomic DNA (bacteria)

Post by daniell » Tue Feb 21, 2006 8:28 am

Is it possible to PCR out a 1.3kb gene from a DNA genomic of 6.18Mb??

sdekivit
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Post by sdekivit » Tue Feb 21, 2006 10:37 am

if you have the correct primers, it could be done.

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canalon
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Post by canalon » Tue Feb 21, 2006 12:47 pm

Good primers, good enzymes, and good conditions, no problem. The size (of the genome) doesn't matter for the polymerase you need to have a few copies of you gene to start with, no matter the quantity of total DNA.
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