PCR of long genomic DNA (bacteria)

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 10
Joined: Tue Feb 21, 2006 8:18 am

PCR of long genomic DNA (bacteria)

Post by daniell » Tue Feb 21, 2006 8:28 am

Is it possible to PCR out a 1.3kb gene from a DNA genomic of 6.18Mb??

King Cobra
King Cobra
Posts: 586
Joined: Sat Jul 30, 2005 7:16 pm
Location: holland

Post by sdekivit » Tue Feb 21, 2006 10:37 am

if you have the correct primers, it could be done.

User avatar
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Tue Feb 21, 2006 12:47 pm

Good primers, good enzymes, and good conditions, no problem. The size (of the genome) doesn't matter for the polymerase you need to have a few copies of you gene to start with, no matter the quantity of total DNA.

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: No registered users and 8 guests