pRSETA

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Shirley
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pRSETA

Post by Shirley » Mon Feb 20, 2006 8:50 am

Hi,
I am using pRSETA vector for cloning. I don't understand why TOP10F' cells are recommended by Invitrogen, instead of TOP10 cells. Why are recA amd endA strain recommended? I can understand that strains with recA+ tend to be more healthy and to grow faster than recA- strains but why endA strain? In fact endA- strain can significantly improve the quality of the plasmid preparations.
Anyone has any ideas why? Thank you.

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