Why do thermocyclers cycle 25-30 times?

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Samo
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Why do thermocyclers cycle 25-30 times?

Post by Samo » Mon Feb 20, 2006 1:04 am

I'm trying to figure this out for my grade 12 biology class. We did an experiment with PCR, unfortunately we don't have any notes at all related to PCR, and the internet is not much help in explaining why. Can someone give me a quick explanation?

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canalon
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Post by canalon » Mon Feb 20, 2006 6:22 pm

Definitely depends on your conditions, I do some pCR with 15 cycles and some with 35 to 40. But why just think how PCR work (doubling of DNA at each cycle, usually 90 to 95% efficiency) and if you make the caqlculations you will just reach the obvious conclusion: to get a whole lot of DNA in the end.
Patrick

Science has proof without any certainty. Creationists have certainty without
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