Identification beteeen bacillus subtillis and bacillus megaterium?

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Hannahbee8262
Garter
Garter
Posts: 1
Joined: Mon Jan 29, 2018 3:03 pm

Identification beteeen bacillus subtillis and bacillus megaterium?

Post by Hannahbee8262 » Mon Jan 29, 2018 3:14 pm

Hi everyone,
I was wondering if someone could possibly help me
I’m in my first year of uni and one of my courses Is for microbiology

I’ve been given bacteria that I need to identify through various tests
I’m stuck between two bacterium for two specimens;

Here are the tests I have done for both specamins done on TSA:

1. It is a creamy white morphology that looks almost fluffy on TSA agar.
Gram: positive
Catalase: positive
Oxidase: negative
Indole test: orange
Under the microscope there are small purple rods gathered in almost what look like clusters in big groups all tightly packed together

2. A white creamy morphology too however they are round singular colonies in shape and have “rings” within each single colony
Gram: positive
Catalase: positive
Oxidase: negative
Indole test: yellowy
Under the microscope they are rods arranged in singular long chains

I feel like the first one is bacillus subtillis and the second one is bacillus megaterium

As they are both positive in the starch test and all the information I can find online says they both hydrolyse protein on milk agar- I have no idea what tests to do In order to be certain which is which?

If anyone could please offer me some help I’ve been losing my mind for a week :? :(

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest