plant dna isolation?

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angel
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plant dna isolation?

Post by angel » Thu Feb 16, 2006 9:54 am

according to standard plant DNA isolation methods, there is not much mention about the interference of mt DNA or ch DNA in PCR amplification. can their presence be ruled out in determining genetic diversity

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Dr.Stein
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Post by Dr.Stein » Mon Feb 20, 2006 6:13 am

mtDNA? chDNA?

Probably a stupid question, yes, but I really am not into molecular-based study so I have no idea :oops:
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canalon
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Post by canalon » Tue Feb 21, 2006 1:19 pm

Mt DNA: Mitochondrial DNA
ch DNA: Chloroplastic DNA.

As for the question, depends of the set of primer you use. If they are specific enough, your mt- and chDNA are just not going to be amplified, so they are not relevant to your results, and yo can go on with diversity study.
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any proof. (Ashley Montague)

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