how does DNA degrade at room tempearture?

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purna
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how does DNA degrade at room tempearture?

Post by purna » Thu Feb 16, 2006 6:29 am

how does DNA degrade at romm temperature?
Please also suggest how i can store DNA at room temperature

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canalon
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Post by canalon » Thu Feb 16, 2006 10:01 pm

DNA degrades mainly because of DNAses. Otherwise it would be rather stable. In a Buffered solution with EDTA to inhibit DNAse you can store DNA at 4ºC for a few days. For stability at RT, I suggest precipitation and drying.
Patrick

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any proof. (Ashley Montague)

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Dr.Stein
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Post by Dr.Stein » Mon Feb 20, 2006 6:48 am

I have a problem, maybe you can help me to analyze and explain me how. I want to read DNA from preserved cells. When I use formaldehyde as a fixative I got nothing to read, you know bands. Then they suggest me to use ethanol for the fixative. Yes, I can see bands pretty well. I want to know why this happens. Thank you.
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canalon
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Post by canalon » Mon Feb 20, 2006 6:17 pm

A quick search on the internet (not with Google though ;)) gave me the answer. Formalin fixation cause crosslinking of DNA and RNA to proteins. More details and some references here
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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