Need Help? Plasmid from Gram(+)

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
cloudnine
Garter
Garter
Posts: 35
Joined: Wed Dec 22, 2004 5:03 am

Need Help? Plasmid from Gram(+)

Post by cloudnine » Tue Feb 14, 2006 3:35 am

I have to do plasmid extraction Bacillus thuringiensis
I have heard that plasmid extraction from Gram(+) bacteria are somewhat different from that from Gram(-).
Can anyone help to tell me what these differences are?

thank you,

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Tue Feb 14, 2006 2:12 pm

Mostly the lysis procedure is different. Those Gram(+) are tougher than the Gram(-). Due to their cell wall. But it also depends on the species. In my lab we work with Enterococcus spp. and Listeria spp. and the latter are much harder to break than the other.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

rexarunraj
Garter
Garter
Posts: 4
Joined: Tue Feb 21, 2006 3:32 pm

Post by rexarunraj » Tue Feb 21, 2006 3:54 pm

try this out ...

1. Boiling method of plasmid isolation

or

2. Lysozyme for lysis of the cell

ali seify nezhad
Garter
Garter
Posts: 1
Joined: Wed Jul 12, 2006 11:29 am

Re: Need Help? Plasmid from Gram(+)

Post by ali seify nezhad » Wed Jul 12, 2006 11:40 am

I have to do plasmid extraction Bacillus thuringiensis
I have heard that plasmid extraction from Gram(+) bacteria are somewhat different from that from Gram(-).
Can anyone help to tell me what these differences are?

thank you [/b][/code][/list]

User avatar
mkwaje
Coral
Coral
Posts: 146
Joined: Mon Jul 03, 2006 2:48 am
Location: Philippines

Post by mkwaje » Thu Jul 13, 2006 9:25 am

I agree with rexarunraj, addition of lysozyme is very helpful in lysis of Gram+ cells.

seaseasea
Garter
Garter
Posts: 20
Joined: Tue May 16, 2006 2:49 am
Location: China

Post by seaseasea » Thu Jul 13, 2006 9:43 am

Yes,addition of lysozyme is very easy.Generally,I put lysozyme in solution I.This method is used in our lab to isolation plasmid from Bacillus subtilis.
Happiness is my favourite!God is also my favourite!
Happiness is also God!

Post Reply

Who is online

Users browsing this forum: No registered users and 3 guests