gel loading buffer

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Shirley
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gel loading buffer

Post by Shirley » Mon Feb 13, 2006 5:44 am

I mixed my samples with 6x gel loading buffer and loaded them onto gel but the mixtures keeping flowing out from the slots. The slots were initially empty. I guess it must be due to my gel loading buffer. Anyone has any ideas what's wrong with my ldye?

Thanks

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canalon
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Post by canalon » Mon Feb 13, 2006 3:40 pm

No, because i can't see it. How did you prepare it?
Washing inside the wells (pipette some buffer in and out) is sometimes useful. Of course oil on your reaction can sometimes cause this effect. But I wasn't here.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

rexarunraj
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Loading dye

Post by rexarunraj » Tue Feb 21, 2006 3:43 pm

there may be two things you should check

1. check for the one which increases the density (sucrose / glycerol)
2. check if there is any air bubble in the slot or while you load

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canalon
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Post by canalon » Tue Feb 21, 2006 5:03 pm

Another thing: was your gel in 1X or 10x TAE (or TBE) I've heard this can lead to this kind of problem...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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