sucrose gradient help

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plant
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Joined: Sat Feb 11, 2006 3:27 pm

sucrose gradient help

Post by plant » Sat Feb 11, 2006 3:30 pm

hi all,

Anybody done sucrose gradient for separating organelles?

Can I use frozen tissue? how much I should use?

I want to separate ER and PM, what kind of condition I should use?

What is other things I have be careful about?

Any lead is welcome

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