about mRNA structure stability

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about mRNA structure stability

Post by imen » Thu Feb 09, 2006 8:11 am

hello everybody,
:D Dear Sir,

i want to compare 2 mRNA structure stability, i work on recombinant protein expression, so i have to check if the corresponding mRNA are stable.
i have used mfold Web-site to determine the mRNA free energy of 2 different proteins. the results are :
1- Séq 1: dG= -127.47 [Initially -140.51]
2- Séq 2: dG= -142.55 [Initialy -155.71]
my questions are:
1- why we have two different enthalpy for a same mRNA, in another word why the results shows 2 dG exp:dG= -127.47 and Initially -140.51
which one i have to take?
2- from which valeur i can say that my mRNA sequence is stabl??
thank you for your precious help!!
if some one can help me to expain my result thanks a lot :oops:

Miss Imen RABHI

King Cobra
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Post by sdekivit » Fri Feb 10, 2006 8:40 am

well, dG doesn't give you the enthalpy ;) --> it's the free Gibbs energy

In general: when the Gibbs free energy is negative a process will be spontaneous. So which one would be the most stable mRNA ? (although it doesn't differ greatly)

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Inland Taipan
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Post by Poison » Sat Feb 11, 2006 3:35 pm

Can somebody explain me the point of double-posting? Please do not.
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How charged with punishment the scroll
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