help please : about mRNA structure stability

[imen]

help please : about mRNA structure stability

• Quote

Post by imen » Wed Feb 08, 2006 5:30 pm

hello everybody,

Dear Sir,

i want to compare 2 mRNA structure stability, i work on recombinant protein expression, so i have to check if the corresponding mRNA are stable.
i have used mfold Web-site to determine the mRNA free energy of 2 different proteins. the results are :
1- Séq 1: dG= -127.47 [Initially -140.51]
2- Séq 2: dG= -142.55 [Initialy -155.71]
my questions are:
1- why we have two different enthalpy for a same mRNA, in another word why the results shows 2 dG exp:dG= -127.47 and Initially -140.51
which one i have to take?
2- from which valeur i can say that my mRNA sequence is stabl??
thank you for your precious help!!
if some one can help me to expain my result thaks a lot

Miss Imen RABHI
from FRANCE

[delgrodel]

Re: help please : about mRNA structure stability

• Quote

Post by delgrodel » Sat Feb 11, 2006 10:17 am

i want to compare 2 mRNA structure stability, i work on recombinant protein expression, so i have to check if the corresponding mRNA are stable.
i have used mfold Web-site to determine the mRNA free energy of 2 different proteins. the results are :
1- Séq 1: dG= -127.47 [Initially -140.51]
2- Séq 2: dG= -142.55 [Initialy -155.71]
my questions are:
1- why we have two different enthalpy for a same mRNA, in another word why the results shows 2 dG exp:dG= -127.47 and Initially -140.51
which one i have to take?
2- from which valeur i can say that my mRNA sequence is stabl??
thank you for your precious help!!
if some one can help me to expain my result thaks a lot

Miss Imen RABHI
from FRANCE

Hi Imen

1. For all intensive purposes, use the values not bound by brackets (-127.47 and -142.55).

2. Both are relatively stable structures. But since the second reaction has a lower deltaG, I suppose you can say the resultant RNA strand is more stable

Hope that helped.