help please : about mRNA structure stability
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help please : about mRNA structure stability
hello everybody,
Dear Sir,
i want to compare 2 mRNA structure stability, i work on recombinant protein expression, so i have to check if the corresponding mRNA are stable.
i have used mfold Web-site to determine the mRNA free energy of 2 different proteins. the results are :
1- Séq 1: dG= -127.47 [Initially -140.51]
2- Séq 2: dG= -142.55 [Initialy -155.71]
my questions are:
1- why we have two different enthalpy for a same mRNA, in another word why the results shows 2 dG exp:dG= -127.47 and Initially -140.51
which one i have to take?
2- from which valeur i can say that my mRNA sequence is stabl??
thank you for your precious help!!
if some one can help me to expain my result
thaks a lot
Miss Imen RABHI
from FRANCE
Dear Sir,
i want to compare 2 mRNA structure stability, i work on recombinant protein expression, so i have to check if the corresponding mRNA are stable.
i have used mfold Web-site to determine the mRNA free energy of 2 different proteins. the results are :
1- Séq 1: dG= -127.47 [Initially -140.51]
2- Séq 2: dG= -142.55 [Initialy -155.71]
my questions are:
1- why we have two different enthalpy for a same mRNA, in another word why the results shows 2 dG exp:dG= -127.47 and Initially -140.51
which one i have to take?
2- from which valeur i can say that my mRNA sequence is stabl??
thank you for your precious help!!
if some one can help me to expain my result


Miss Imen RABHI
from FRANCE
Re: help please : about mRNA structure stability
imen wrote:i want to compare 2 mRNA structure stability, i work on recombinant protein expression, so i have to check if the corresponding mRNA are stable.
i have used mfold Web-site to determine the mRNA free energy of 2 different proteins. the results are :
1- Séq 1: dG= -127.47 [Initially -140.51]
2- Séq 2: dG= -142.55 [Initialy -155.71]
my questions are:
1- why we have two different enthalpy for a same mRNA, in another word why the results shows 2 dG exp:dG= -127.47 and Initially -140.51
which one i have to take?
2- from which valeur i can say that my mRNA sequence is stabl??
thank you for your precious help!!
if some one can help me to expain my resultthaks a lot
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Miss Imen RABHI
from FRANCE
Hi Imen

1. For all intensive purposes, use the values not bound by brackets (-127.47 and -142.55).
2. Both are relatively stable structures. But since the second reaction has a lower deltaG, I suppose you can say the resultant RNA strand is more stable

Hope that helped.
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