Buffer concentration

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LaurieCate
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Joined: Sat Feb 04, 2006 7:54 pm

Buffer concentration

Post by LaurieCate » Tue Feb 07, 2006 3:02 pm

The components of a typical buffer are as follows:

100 mM Tris, pH 7.5
50 mM NaCl
10 mM MgCl2
1 mM dithiothreitol (DTT)
100 micrograms/milliliter BSA

The above buffer is 1X (1-times concentrated). I need to write a recipe for a 10X-concentrated buffer for the enzyme buffer above.

____ mM Tris, pH 7.5
____ mM NaCl
____ mM MgCl2
____ mM dithiothreitol (DTT)
____ micrograms/milliliter BSA

How would I go about solving this problem? Should certain components (such as DTT and BSA) be increased 10 fold? I am at a loss.

sdekivit
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Re: Buffer concentration

Post by sdekivit » Tue Feb 07, 2006 3:12 pm

LaurieCate wrote:The components of a typical buffer are as follows:

100 mM Tris, pH 7.5
50 mM NaCl
10 mM MgCl2
1 mM dithiothreitol (DTT)
100 micrograms/milliliter BSA

The above buffer is 1X (1-times concentrated). I need to write a recipe for a 10X-concentrated buffer for the enzyme buffer above.

____ mM Tris, pH 7.5
____ mM NaCl
____ mM MgCl2
____ mM dithiothreitol (DTT)
____ micrograms/milliliter BSA

How would I go about solving this problem? Should certain components (such as DTT and BSA) be increased 10 fold? I am at a loss.


concentration muliplied by 10

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