Magnesium Chloride & Taq polymerase

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boydog
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Magnesium Chloride & Taq polymerase

Post by boydog » Sun Feb 05, 2006 12:49 pm

hi, my teacher told us that Magnesium Chloride is a cofactor of Taq polymerase i nthe process of PCR. And when we asked what does Magnesium chloride do to Taq polymerase, she told us to look for it online. But i can't find it.

Cofactor: any substance that needs to be present in addition to an enzyme to catalyze a certain reaction.

So what exactly does Magnesium Chloride do?

sdekivit
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Post by sdekivit » Tue Feb 07, 2006 3:14 pm

the magnesium is the cofactor for the enzymeand it weill probably arrnge the protein in the right conformation so that the enzyme is fully active.

boydog
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Post by boydog » Wed Feb 08, 2006 11:46 am

so it arranges Taq polymerase in the right conformation usch that Tq polymerase is fully active??

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