Carbonylation

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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isiisi
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Carbonylation

Post by isiisi » Fri Jun 23, 2017 7:26 am

Hello,

I'm a junior reseacher and I'm right now trying to measure the carbonylation of a specific protein (htz1p in s.cerevisiae). I think i should use DNH since it is specific to keton/aldehyd groups to measure the carbonylation. I'm however not sure how to best separate my protein and how to prevent artificial oxidation during the analysis. Does anyon have any tipps or maybe an article that they can recommend me?

PS: My lab can't do a 2D electrophorese.

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