DNA restriction sites/fragments calculation?

Genetics as it applies to evolution, molecular biology, and medical aspects.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
apronedsamurai
Garter
Garter
Posts: 1
Joined: Thu Jun 16, 2016 10:16 am

DNA restriction sites/fragments calculation?

Post by apronedsamurai » Thu Jun 16, 2016 10:20 am

What types of DNA fragments would result if you were to cut the plasmid shown with both restriction enzymes, EcoR1 and BamH1?

a. 1/2 20 kb, 1/4 30 kb, 1/4 10 kb
b. 1/2 20 kb, 1/2 60 kb
c. 1/2 30 kb, 1/2 50 kb
d. All 80 kb



Image

I have identified the answer as being a.

My reasoning is this:

Distance between EcoR1 (20kb) and BamH1 (40kb) =20 kb

Distance between BamH1 (40kb) and BamH1(70kb)=30kb

Distance between BamH1 (70kb) and EcoR1 (80kb) =10kb

What I am confused by though, is the 1/2 and 1/4 component of the answer given. Can anyone give me a breakdown of that please?

Fornita
Garter
Garter
Posts: 43
Joined: Fri May 13, 2016 9:07 am

Post by Fornita » Fri Jun 17, 2016 3:55 am

Your image did not show correctly, could you post again?
Andrea Fortina
Genius only means hard-working all one's life.
CreativeBiomart, Recombinant protein expert

Post Reply

Who is online

Users browsing this forum: No registered users and 3 guests