Microbiome and diseases

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Zeynel
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Microbiome and diseases

Post by Zeynel » Tue Nov 10, 2015 4:43 pm

Hello, I hope this is the right forum to ask this question. I'm a layman who is very interested in the microbes that live in our gut. I want to initiate a citizen science project to relate gut microbes to known diseases. For instance, this research ties Lupus to gut microbes http://www.hcplive.com/conference-coverage/acr-2015/microbiome-seems-to-have-role-in-triggering-lupus and this research ties asthma to gut microbes http://www.health24.com/Diet-and-nutrition/Nutrition-basics/Link-between-gut-bacteria-asthma-and-anorexia-20151105

I'm imagining to form a company like ubiome http://ubiome.com/ .

My question is: What type of machinery ubiome or anyone who wants to identify gut microbes uses? I'm in Turkey and I want to find out if this test can be done here.

My other question is: It is possible to identify gut microbes by traditional lab methods as in a Comprehensive Stool Test without using DNA. İs this technique inferior to DNA method?

I contacted ubiome and also American Gut Project http://humanfoodproject.com/americangut/ for answers to these questions but they never answered me. I wrote to several academics and they never replied either.

Also please remember that I know nothing about microbiology.

Thanks.

Asiaferret
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Post by Asiaferret » Sat Dec 26, 2015 9:20 am

Stool examination can check for pathogens that causes infections to the digestive system (especially the small intestine and colon). Examples are enteric bacteria that causes diarrhea such as Salmonella typhi and certain strains of Escherichia coli along with other organisms which may be eukaryotic. In this article http://aem.asm.org/content/74/5/1469.full it is described on how faeces samples are collected, dissolved in some solvent, have its components separated in a centrifuge and the relevant molecules are analysed with High performance liquid chromatography for comparison. DNA sequencing however can provide accurate data about an organism's genetic makeup (unique for each species) and even certain mutations or single nucleotide polymorphism. DNA sequencing has a number of methods such as Chain termination method and chemical cleavage method. DNA will provide the most definitive answer regarding an organism's species and subtypes and even evolutionary relationship. I may be wrong. Check with other sources.

leesajohnson
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Post by leesajohnson » Fri Sep 09, 2016 9:18 am

The microbiome varies dramatically from one individual to the next and can change quickly over time in a single individual. The great majority of the microbes live in the gut, particularly the large intestine, which serves as an anaerobic digestion chamber. Scientists are still in the early stages of exploring the gut microbiome, but a burgeoning body of research suggests that the makeup of this complex microbial ecosystem is closely linked with our immune function. The microbiome is billions of microorganisms that live inside of us. There are estimated to be 10 times as many cells in us that aren't us as there are cells that are us. However, these cells are far smaller than our cells. We couldn't live without them. They help us carry out many important digestive functions. http://www.medscape.com/viewarticle/812353
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418802/

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