enzymatic digestion of rna' s

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naruto
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enzymatic digestion of rna' s

Post by naruto » Wed Jan 18, 2006 12:59 pm

I am trying to digest nucleotides (>200) enzymatically.Any suggestions about the most effective enzymes,that i can use in order to obtain small fragments?

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victor
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Post by victor » Wed Jan 18, 2006 1:04 pm

use endonuclease restrictions.....there're EcoR1 and friends...
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MrMistery
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Post by MrMistery » Thu Jan 19, 2006 8:22 pm

Be advised: each restriction endonuclease has a specific binding site for a special sequence of nucleotides
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canalon
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Post by canalon » Thu Jan 19, 2006 8:49 pm

Try a short cutter (usually 4 are used like RsaI the fragments are in the few hundreds base long) or something with lots of N. Have a look on rebase. But <200 base, that is really small.
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