Assistant Project Scientist Step I- Step VI -Neuroscience -

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Assistant Project Scientist Step I- Step VI -Neuroscience -

Post by UCberkeley » Thu Jul 30, 2015 10:25 pm

University of California, Berkeley

Assistant Project Scientist Step I- Step VI -Neuroscience - Molecular and Cell Biology

Recruitment Period
Open date: July 23rd, 2015 Next review date: August 7th, 2015
Apply by this date to ensure full consideration by the committee. Final date: August 24th, 2015
Applications will continue to be accepted until this date, but those received after the review date will only be considered if the position has not yet been filled.


We are seeking a highly motivated individual to carry out original research using state-of-the-art single cell genomics and CRISPR-Cas9 genome editing technologies, with the goal of characterizing cellular diversity in the mammalian brain. The successful candidate will use high-throughput single cell RNA-sequencing methodologies to classify cells based on their transcriptome profiles, and CRISPR-Cas9 genome engineering techniques to genetically label newly discovered cell types in transgenic models. As part of an NIH-funded BRAIN Initiative project that includes 10 research groups and 3 technology core facilities on the UC Berkeley campus, this individual will be an integral member of a dynamic, multidisciplinary team of neuroscientists, molecular biologists, genomics technologists, mouse geneticists, statisticians and computer scientists to develop and deploy new technologies for creating a taxonomy of cell types in the brain.


The successful candidate will have primary responsibility for managing the project's transgenic mouse colony; isolating tissue from defined brain regions; isolating preparations of viable neurons by fluorescence-activated cell sorting (FACS) and related techniques; capturing single cells using FACS and/or microfluidics techniques; preparing libraries for Illumina sequencing; participating in bioinformatics analysis of DNA/RNA-sequencing data; conducting qRT-PCR, immunohistochemistry and RNA in situ hybridizations; designing and constructing targeting constructs for CRISPR-Cas9 genome engineering; and creating transgenic mice using embryonic stem cell (ESC) and CRISPR-Cas9 technologies.

Minimum/Basic Qualifications Required (By the time of application):

Applicants must possess a PhD or equivalent degree in neuroscience, molecular biology, developmental biology or a related field.

Additional Required Qualifications (By start date):

Hands-on experience and demonstrable expertise in routine molecular biology (molecular cloning, PCR, plasmid design and construction); histochemical techniques including multi-label immunohistochemistry and RNA in situ hybridization; library preparation and quality control for Illumina high-throughput DNA sequencing; vibratome sectioning of live brain tissue; isolation of viable neurons from mouse sensory cortex; purification of viable cortical neurons by FACS; microfluidic capture and processing of viable cortical neurons for RNA-sequencing using the Fluidigm C1 or similar platform; mouse genetics and experimental embryology; homologous recombination in ESCs or mice embryos using CRISPR-Cas9 genome engineering; ESC transfection and culture.

Preferred Qualifications (By start date):

Working knowledge in mammalian neuroanatomy and bioinformatics analysis; experience in supervising technical staff.


This position reports to Dr. John Ngai. The initial appointment will be for 100% time for 1 year with the expectation of extension based on availability of funds. The approximate start date for this position will be November 2015.

Salary: $53,100-$68,800 depending on qualifications. This position provides full benefits.

To Apply:

Interested individuals should submit application documents as PDFs, which includes, an updated curriculum vitae, and names with contact information for at least 3-5 individuals who have agreed to provide a reference for this specific position and a cover letter. (Letters of reference may be requested of the finalists).

Department Contact: Shannon Kotter, Senior HR Partner. mailto:[email protected] This recruitment will remain open until filled.
All letters will be treated as confidential per University of California policy and California state law. Please refer potential referees, including when letters are provided via a third party (i.e., dossier service or career center), to the UC Berkeley statement of confidentiality ( prior to submitting their letters.

The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy see:
The department is interested in candidates who will contribute to diversity and equal opportunity in higher education through their work.


Updated Curriculum Vitae

Cover Letter

References3-5 references required (contact information only)


To apply, visit:

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