Electrophoresis

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Dr.Hawaii
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Electrophoresis

Post by Dr.Hawaii » Sun Jan 15, 2006 12:41 am

how would raising the voltage affect the results of electrophoresis? How would lowering?

Also how does the polarity affect electrophoresis?

sdekivit
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Post by sdekivit » Sun Jan 15, 2006 9:14 am

what is the property of the separation with electrophoresis? Molecular weight. In order to separate, you need samples with a negative charge, so polarity have only a very little contribution in the separation.

--> when u raise the voltage, the samples will migrate faster. But you must be aware of rising of the temperature of the gel ;)

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Dr.Stein
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Post by Dr.Stein » Mon Jan 16, 2006 12:49 am

Aee there is another Doctor here... hello :D
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