Mutants and knockdowns differ in compensation response

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: Leonid, amiradm, BioTeam

Post Reply
User avatar
jonmoulton
Viper
Viper
Posts: 442
Joined: Fri Feb 15, 2008 5:38 pm
Location: Philomath, Oregon, USA

Mutants and knockdowns differ in compensation response

Post by jonmoulton » Mon Jul 13, 2015 4:29 pm

If you want to knock down a gene to learn about its function, it can be useful to assess the loss of function without a background of altered gene expression due to compensation. Now there are data showing that mutants can compensate and the response differs from that of morphants.

Rossi A, Kontarakis Z, Gerri C, Nolte H, Hölper S, Krüger M, Stainier DYR. Genetic compensation induced by deleterious mutations but not gene knockdowns. Nature. 2015;[Epub ahead of print] doi:10.1038/nature14580
http://www.nature.com/nature/journal/vaop/ncurrent/full/nature14580.html


Hesman Saey T. Mutation-disease link masked in zebrafish. ScienceNews. 2015;July 13
https://www.sciencenews.org/article/mutation-disease-link-masked-zebrafish

bocsciences2005
Garter
Garter
Posts: 2
Joined: Tue Jul 14, 2015 3:46 am

Post by bocsciences2005 » Tue Jul 14, 2015 4:01 am

Nice suggestion to learn about the function of a gene. By the way you suggested, the learning process will be more interesting and there will be more possibilities for the discovery of unknown. Thanks for the sharing!-BOC Sciences Screening Libraries ( http://www.bocsci.com/Screening-Libraries.asp )

Post Reply

Who is online

Users browsing this forum: No registered users and 4 guests