Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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I want to measure a protein in Bronchoalveolara Lavage Fluid (BALf) which is the lining fluid from the bronchial and alveolar epithelium. It is aspirated with saline through Bronchoscope. I am going to do elisa, which i get from here (http://www.creative-diagnostics.com/Con ... pment.html), but i don't know what is the point of measuring the total protein concentration before Elisa. The most papers i have reviewed, using Elisa in BALf, have results in pg/ml or ng/ml or ug/ml and they have not performed an assay for the total protein concentration.
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