Why cells dies in flow cytometry analysis??

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w19910411
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Why cells dies in flow cytometry analysis??

Post by w19910411 » Wed Mar 11, 2015 8:46 am

Why are all my cells in flow cytometry analysis dead??

Fornita
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Post by Fornita » Mon Jun 13, 2016 9:45 am

Your operation process, the temperature, all these could influence the cell, especially your treatment, which does not gentle enough.
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biomind2017
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Re: Why cells dies in flow cytometry analysis??

Post by biomind2017 » Tue Jan 24, 2017 9:47 am

Recently, I used FACS-buffer (PBS, 2 mM EDTA, 25 mM HEPES) for antibody dilution and washing. Although, clump formation was less but it was present. Besides, I would also like to know that do the presence of HEPES buffer and EDTA interfere with the result while making antibody dilution buffer.

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