Questions about DNA dialysis

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protoemics
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Questions about DNA dialysis

Post by protoemics » Thu Feb 05, 2015 7:56 am

Dear all,
I would like to use dialysis to exchange ions in my DNA. For this I bought Slide-A-Lyzer mini pots for the dialysis.
First, I am looking for more information about the theoretical background of dialysis. Can you give me some literature hints, please?

Secondly, I know my target DNA concentration, but I know dialysis can lead to dilution and sample loss. How much higher shall I choose the starting concentration?
Also, how can I calculate the optimum time for dialysis, the right temperature, please?

Thank you very much for your hints.

leesajohnson
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Post by leesajohnson » Tue Sep 13, 2016 8:28 am

Kidney function goes down and the body is not able to remove waste and excess water from the blood, dialysis is needed.
In dialysis the waste & excess water is removed from the body by using an artificial kidney. During kidney failure, dialysis is a life-support treatment that uses a special machine to filter harmful wastes, salt, and excess fluid from your blood. Dialysis replaces many of the kidney's important functions. It is typically a 3-4 hour process where blood is taken out from the body and passed through dialyser where the blood is filtered and then put back in the body. These processes can be done in a hospital or at home.
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KikoGarcia
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Re: Questions about DNA dialysis

Post by KikoGarcia » Wed Jan 23, 2019 9:13 am

De novo Sequencing can be used to sequence uncharacterized genomes, if there is no reference sequence available, or known genomes if significant structural variation is expected.
The general strategy of de novo sequencing analysis is to align and merge short fragments derived from a much longer DNA sequence in order to reconstruct the original sequence. de novo sequencing projects usually take multiple libraries and multiple rounds of finishing to get a complete genome sequence.
Sourse: DNA data analysis.

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