cell culture technique

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angel92
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cell culture technique

Post by angel92 » Sun Dec 07, 2014 8:41 pm

You trypsinize a T75 flask of CHO cells and resuspend your cells with media (15mL total). You determine your cell suspension contains 5 x 106 cells/ml. Howmany mLs of cells/media will you need to subculture a new flask containing:
a.50 mL of medium at a final concentration of 1.5 x 105 cells/mL?
b.140 mL of medium at a final concentration of 2.25 x 105 cells/mL?

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