Swarming Assay

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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SpellForce
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Swarming Assay

Post by SpellForce » Wed Oct 29, 2014 8:21 pm

Hi,

I have a question considering a swarming assay on E.coli...
I did a soft agar (0.3%), in LB, and placed a drop of bacteria in the middle of a plate. But after 24h, they just grew in a small radius, like in the photo:
Image

DO you think I have non-motile E.coli? Cause I would expect a bigger circle. Or Im just doing something wrong...
How is this different from a swimming assay?

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