Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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From what I understand, the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression clone of a certain gene? Why can't I use an attL-PCR product or a linearized attL DNA and make a reaction with the destination vector, therefore obtaining the expression clone attB in the end? I can't understand why we have to perform the first reaction! Help!
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