Plasmid yield/concentration

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Helics
Garter
Garter
Posts: 42
Joined: Fri Oct 12, 2012 9:10 am

Plasmid yield/concentration

Post by Helics » Thu Jul 31, 2014 3:40 pm

What is the difference between high yield plasmid and high concentration plasmid ?

Helics
Garter
Garter
Posts: 42
Joined: Fri Oct 12, 2012 9:10 am

Re: Plasmid yield/concentration

Post by Helics » Sat Aug 02, 2014 3:55 am

Dear, scientist
please give a response.

Cat
King Cobra
King Cobra
Posts: 635
Joined: Thu Feb 14, 2008 7:40 pm

Post by Cat » Sat Sep 20, 2014 4:18 pm

"High yield plasmid" means that host cells make a lot of copies per cell and that usually results in high plasmid concentration in the final DNA preparation. Alternatively, larger volume of stating material to smaller volume elution can be used to concentrate low yield plasmid prep...

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Mon Sep 22, 2014 7:48 am

I think neither of those is used. Plasmids are called either high copy or low copy depending on number of copies per cell.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest