Centrifuges and Electrophoresis self-made?

Discussion of all aspects of cellular structure, physiology and communication.

Moderators: Leonid, amiradm, BioTeam

Post Reply
Ultrashogun
Coral
Coral
Posts: 149
Joined: Wed Jan 04, 2006 4:48 am

Centrifuges and Electrophoresis self-made?

Post by Ultrashogun » Wed Jan 04, 2006 5:16 am

My question is probably really naive, but is it possible to make the apparatus for electrophoresis and centrifuges in an affordable way? For electrophoresis it should be possible quite easily.

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Wed Jan 04, 2006 9:51 pm

If you find out, let me know...
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

User avatar
Poison
Inland Taipan
Inland Taipan
Posts: 2322
Joined: Sun Jan 02, 2005 12:44 pm
Location: Turkey

Post by Poison » Thu Jan 05, 2006 9:50 pm

How can you do that? I couldn't get it.
It matters not how strait the gate
How charged with punishment the scroll
I am the Master of my fate
I am the Captain of my soul.

bearhug
Coral
Coral
Posts: 103
Joined: Thu Jan 05, 2006 10:06 pm
Location: GA

Post by bearhug » Thu Jan 05, 2006 10:26 pm

Electrophoresis and centrifuges I would imagine are pretty hard to make affordably. You use a mutagen for gel electrophoresis (I forget the name but I believe it begins with an A) which you can't get just anywhere.
"In omnias paratus!"

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Fri Jan 06, 2006 9:24 pm

etidium bromure
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

bearhug
Coral
Coral
Posts: 103
Joined: Thu Jan 05, 2006 10:06 pm
Location: GA

Post by bearhug » Sun Jan 08, 2006 8:31 pm

ethidium bromide , yes that's what it is
"In omnias paratus!"

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Mon Jan 09, 2006 4:03 pm

Well making a tank for electrophoresis is probably not too hard if you are good at this kind of things, the power generator can be a bit trickier. And as it has been said the reagent used to prepare and stain gels (agarose and EtBr or other less toxic, but rather more expensive) will be the real big problem.
For the centrifuges they were artisanally built long ago, but due to safety reason, and cost it is usually easier to buy one.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

DevGrp
Death Adder
Death Adder
Posts: 82
Joined: Wed Jan 26, 2005 12:32 pm
Location: UK

Post by DevGrp » Wed Jan 11, 2006 12:58 pm

Home made Electrophoresis Gel tanks

I've worked in a number of labs with home made DNA and protein gel tanks, usually made with perspex / glass and silver wire for the electrodes. The power supply is a bit tricker but i imagine its possible to get an AC to DC transformer with a high enough current output.

With a credit card I would imagine you can buy EtBr / Agarose from most suppliers (eg Sigma) either over the phone or online. These days we use Sybersafe which is an alternative apparently "safer" dye. Finally (for DNA) you would need a UV lamp to allow you see the DNA. You want something with a wavelength around 300nm.

a quick google search found this article which might be of interest (we don't have a subscription though)
Sepel, Lenira M. N., Loreto, Elgion L. S.
Isolation and Visualization of Nucleic Acid with Homemade Apparatus: Practical Activities for Secondary Schools
Biochemistry and Molecular Biology Education 2002 30: 306-308

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest