Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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- Posts: 6
- Joined: Wed Aug 03, 2011 7:51 am
I want to check the 5' LTR sequence of my plasmid. I will like to confirm that it is a hybrid 5' LTR and not a wild type HIV-1 5' LTR. Can someone pls guide me on how I can evaluate this?
- Posts: 42
- Joined: Fri Oct 12, 2012 9:10 am
You may try to find comparable restriction endoneucleases for wild type 5'LTR.
Hybrid 5'LTR generates different
size RE digest products.
- Posts: 7
- Joined: Fri Oct 26, 2012 12:37 am
well, are there many different wild types out there? I mean, why not sequencing to find it out?
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