Determine whether gene lac I and lac O is functioning?

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eos
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Determine whether gene lac I and lac O is functioning?

Post by eos » Fri May 09, 2014 11:57 am

My sample is treated in 3 ways: No inducer, with lactose , with IPTG. ONPG is used in all sample to determine the beta galactosidase. I know that both lactose and IPTG can associate with lac repressor and cause expression. If the result of these three treatment only with IPTG appears yellow, can you deduce lac I and lac O which is functioning or not?

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