Enzymes

Human Anatomy, Physiology, and Medicine. Anything human!

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UkranianKid
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Enzymes

Post by UkranianKid » Thu Dec 15, 2005 9:23 pm

I have had some trouble finding an enzyme to expirement with as in a project for school. My objective is to find what makes Enzyme productivity less or more using pH, temperature, and other factors. I cannot think of one right now as I am a little slow today but I need the answer today hopefully and turned to you guys here on these forums for help.

Could someone please give me an enzyme easy to find and that won't be too much of a hassle to get more of more further examinations.

Thank you for your time, Eugene.

memphis1953
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enzyme lab

Post by memphis1953 » Fri Dec 16, 2005 4:03 am

enzyme: catalase
substrate: 3% hydrogen peroxide solution

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Post by UkranianKid » Thu Dec 22, 2005 3:28 am

I still need another Enzyme, anyone else at all. I'll take as many of them as you have. Please help project due on Friday and I still have to get to the laboratory.

Eugene

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canalon
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Post by canalon » Thu Dec 22, 2005 4:11 am

enzyme: salivary amylase
substrate: starch solution.
Patrick

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Poison
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Post by Poison » Thu Dec 22, 2005 6:54 am

Use some liver. It is the easiest. Saliva is another choice of course.
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Enzyme

Post by JB » Fri Dec 23, 2005 1:43 am

In Pancreas:
pancreatic amylase:Continue digestion of carbohydrates from the mouth.
chymotrypsin:proteins to amino acids
lipase:lipids------glycerol+fatty acids
renin---milk proteins to amino acids :D

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Post by UkranianKid » Sat Dec 24, 2005 1:50 am

I like the saliva one could someone tell me more about that how can siliva be used. Or shown that it has been used anyways.

Eugene

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Post by JB » Sat Dec 24, 2005 4:30 am

saliva is mainy used to dissolve the food particles for digestion, making food particles easier to swallow! :D

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canalon
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Post by canalon » Sat Dec 24, 2005 4:40 am

UkranianKid wrote:I like the saliva one could someone tell me more about that how can siliva be used. Or shown that it has been used anyways.

Eugene


Sorry I don't have any protocol. I guess that a starch solution could mixed with saliva at different temperature and changing pH could do the trick. Detection could be done by seing the increasing the quantity of reducing sugars in the solution.
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MrMistery
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Post by MrMistery » Sat Dec 24, 2005 4:50 pm

Damn and i gave my teacher back her books with practical experiments last month! I actually did the experiment.
Here is the infuence of temperature on salivary amilase:
Take two test-tubes and two pots of water. Heat the water in one pot at about 36-38 degrees celsius(the optimal temperature for ptialine.)and keep the water in the other pot at 0 degrees celsius. Then add starch(you can use bread) into both test tubes and insert them into the pots of water. Let them there for 15 minutes. After that, add some KI solution(lugol) to both test tubes. If you did the experiment correctly the color should be more intense in the one left at 0 degrees as there is more starch left(lugol stains starch blue).
As for other factor, just use your imagination... PS: The optimal pH is about neutral(7)
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Poison
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Post by Poison » Sat Dec 24, 2005 8:14 pm

But, believe me, liver is much more clean, fast and easy. :)
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Post by MrMistery » Sun Dec 25, 2005 4:38 pm

and smells a lot worse too. and i am curios what you might do with liver if you need an experiment on enzymes. the only experiment i ever did with liver is the one with the gall and cooking oil.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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