Debate and discussion of any biological questions not pertaining to a particular topic.
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- Posts: 2
- Joined: Tue Mar 11, 2014 7:13 am
For linirization of plasmid yeast(cervizea)why NotI is used instead of NHEI?
please compare and describe them
- Inland Taipan
- Posts: 5694
- Joined: Mon Sep 14, 2009 7:12 pm
Cis or trans? That's what matters.
- Posts: 5
- Joined: Wed Nov 20, 2013 8:19 am
you must select enzyme that cut your plasmid in one (or two) determined sequense and also not your target gene.
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