Finding RF?

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kitty2
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Finding RF?

Post by kitty2 » Wed Dec 14, 2005 11:10 pm

I got to know this for my final exam.

If I have a number of base pair, how do I find the RF?

How does people do that?
I purpose for asking is so that I can learn it. It is only going to cost me 2 points of my grade if I don't answer this but I care to learn.

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canalon
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Post by canalon » Wed Dec 14, 2005 11:31 pm

Since the Rf depends on each gel (agarose concentration, voltage, buffer and time) there is no other way than running a standard and then plotting Log10(Bp) for each band of the standard vs their Rf. Then either you calculate the equation or you just read on the graph.
Patrick

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any proof. (Ashley Montague)

sdekivit
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Post by sdekivit » Thu Dec 15, 2005 7:58 am

the distance a molecule travels in a gel is inversely proportional to the logarithm of the molecular weight.

--> so you can plot a curve where distance is set out against the logarithm of the molecular weigth and you will get a straight line.

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