aflp plants

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Death Adder
Death Adder
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Joined: Thu Dec 08, 2005 8:31 am

aflp plants

Post by angel » Thu Dec 08, 2005 8:35 am

i am working on rapd fingerprinting of a polysaccharide rich plant. now i want to do an AFLP fingerprint on it. using agarose gel , will it give me good results . also i want to know about designing primers for RE adaptors. plz suggest me some references

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Inland Taipan
Inland Taipan
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Post by canalon » Thu Dec 08, 2005 4:06 pm

try Agricola or Pubmed
to make your bibliography.

As for AFLP, I'm doing that on bacteria with agarose gels. Protocols depends on the length of amplicons, so each protocol ask for it's own optimization. But I am running agarose gels (1.5% for 16 hours to get a good resolution between 300bp to 5000bp)

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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