plasmid extraction on 1.5% agarose gel

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
cah2010
Garter
Garter
Posts: 4
Joined: Wed Dec 18, 2013 1:24 am

plasmid extraction on 1.5% agarose gel

Post by cah2010 » Wed Dec 18, 2013 2:23 am

So I have two bands, the smaller that is around 8-9kb based on the marker ( my plasmids should be 8-9 kb), what is the upper band? Also I have three plasmids and I did this in a set of four replicates
Attachments
IMG_1767.jpg

cah2010
Garter
Garter
Posts: 4
Joined: Wed Dec 18, 2013 1:24 am

Post by cah2010 » Wed Dec 18, 2013 2:27 am

NOTES: bottom is RNA, I didn't have RNAase. First four are should be 7.5KB, last 8 should be 8.8KB like the my markers show.

cah2010
Garter
Garter
Posts: 4
Joined: Wed Dec 18, 2013 1:24 am

Post by cah2010 » Wed Dec 18, 2013 2:30 am

I think that I have the supercoiled forum and nicked form.

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Wed Dec 18, 2013 3:02 pm

is the plasmid treated with anything or is it just after isolation?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

cah2010
Garter
Garter
Posts: 4
Joined: Wed Dec 18, 2013 1:24 am

Re:

Post by cah2010 » Wed Dec 18, 2013 3:37 pm

JackBean wrote:is the plasmid treated with anything or is it just after isolation?


This is just isolation

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Thu Dec 26, 2013 5:56 pm

http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest