dna sequence( prokaryote)

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undrgrad08
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dna sequence( prokaryote)

Post by undrgrad08 » Thu Dec 01, 2005 11:23 pm

i know that if you have a dna sequence that you have to do transcription to get the rna sequence and then do translation. my question is howcan you figure out where the protein synthesis starts and ends?


thanks.

sdekivit
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Post by sdekivit » Fri Dec 02, 2005 7:33 am

there has been done a very simple experiment:

We have a synthetic piece of DNA containing only alanine and at the beginning a lysine.

--> if the sequence was 5' - lys - Ala - Ala - Ala - Ala - 3' then the aminoacid sequence that has been formed was:

N-terminal - Lys - Ala - Ala - Ala - Ala - C-terminal

--> if the sequence was lys at the end, lys would be the C-terminal aminoacid

--> translation always begins at the 5' side.

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