Oxygen consumption

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Mila
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Oxygen consumption

Post by Mila » Fri Oct 11, 2013 12:19 pm

Hello everyone.

I was reading some information about oxygenation effects on cell cultures and I found the following:

"Mammalian cells consume oxygen at rate of 0.05-0.5micromol/10^6 cells/hour. Cell cultures for tissue engineering have relatively large cell densities (10^6 cells/mL) which results in total oxygen depletion in 0.4-4 hours."

Could anyone explain to me how they calculated the time required for cells to consume all the oxygen?

Mila
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Post by Mila » Fri Oct 11, 2013 12:48 pm

I forgot to add that air-saturated aqueous media contains 21mM oxygen

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JackBean
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Post by JackBean » Sat Oct 12, 2013 6:04 pm

You answered it by yourself. You have number of cells, how much oxygen they need and how much oxygen there is. That should be an easy math.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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