hybridization probe

Genetics as it applies to evolution, molecular biology, and medical aspects.

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FDHeLiX
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hybridization probe

Post by FDHeLiX » Mon Sep 16, 2013 3:33 pm

I will use lightcycler 2.0. to detect and SNP with hybridization probes. I designed the probes but I couldn't choose fluorescent dyes, and don't know the criterias. Could you please help me...

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JackBean
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Post by JackBean » Tue Sep 17, 2013 3:43 pm

I'd say it depends solely on your possibilities with the machine (excitation and detection range), ootherwise it shouldn't matter much.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Cat
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Post by Cat » Sun Sep 22, 2013 3:37 pm

JackBean is right, it mostly depends on your machine. However, you need to make sure that fluorophore spectra do not overlap much. You can use this link to help you with that:

http://www.lifetechnologies.com/us/en/h ... iewer.html

You can try this pair - Fluorescein (FITC)/ROX (carboxy-X-rhodamine).

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