About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.
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I started cultivation of E coli JM 109 in innova 4000 Benchtop Incubator shaker at 5.00 pm. E coli were shaken overnight at 170 rpm and 37 degree celsia. Now I am at home and read, that we have always shaken E coli at 150 rpm overnight. Please could the cultivation at 170 rpm overnight damage my E coli or the incubator. I am affraid of overheating the incubator. Should I stop it ? Thank you very much.
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