Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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- Posts: 1
- Joined: Tue Aug 27, 2013 4:19 pm
So I did a double digest for subcloning and I didn't get two distinct bands when I ran the gel. Can someone interpret the gel pattern for me?
- pI-SceI site digest.jpg
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- Inland Taipan
- Posts: 5694
- Joined: Mon Sep 14, 2009 7:12 pm
not really, because there is hardly something visible. What sizes is it? What sizes did you expect?
Cis or trans? That's what matters.
- Posts: 44
- Joined: Sun May 08, 2011 3:26 am
- Location: Kobe, Japan
size of your vector/insert.
gel is prestained with ethidium bromide or not.
what are the restriction enzymes you are using?
did you check if your vector contains multiple sites of the enzymes you are using?
The bands are way up, higher than 10k bp..
AHH, Gravity! Such a cold cruel mistress.
- King Cobra
- Posts: 635
- Joined: Thu Feb 14, 2008 7:40 pm
It looks like a bad gel. I suggest to re-run with fresh buffer/gel.
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