How to store bacteria?

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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rinchan
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How to store bacteria?

Post by rinchan » Mon Nov 21, 2005 12:10 am

who knows that? like storing E.coli. I need details. thanks.

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GreenDog
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Post by GreenDog » Mon Nov 21, 2005 11:23 am

Freeze them in Glycerol in -80.

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canalon
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Post by canalon » Mon Nov 21, 2005 2:31 pm

yeah grow them to early stationnary phase (overnight culture) and freze them in a solution containing at last 15% glycerol. adjusting salt concentration so that that the final concentration is iso-osmotic can increase recovery rate.
Freezing at -80ºC is best, but storage at -20ºC is okay for a few month.

If you can't freeze, store in conservation media in deep agar (I don't have recipe but you should find this in any microbiology textbook I guess)
Patrick

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any proof. (Ashley Montague)

scottyiu
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Post by scottyiu » Thu Dec 08, 2005 9:46 am

doesn't bacteria and fungi die of low temperature?
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GreenDog
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Post by GreenDog » Thu Dec 08, 2005 3:42 pm

No, they undrgo growth arrest. And Glycerol prevents them from bursting out when you thaw them.
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scottyiu
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Post by scottyiu » Fri Dec 09, 2005 1:51 pm

But how can they survive under such low temperture?
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MrMistery
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Post by MrMistery » Fri Dec 09, 2005 7:09 pm

Reduce their methabolism to almost 0...
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