Protons in E.coli???????????

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Lehigh_2007
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Protons in E.coli???????????

Post by Lehigh_2007 » Sun Nov 13, 2005 4:29 am

Hi, I am a student at Lehigh University and am new to the forum, this is my first post. This past Friday my biology teacher offered our class a bonus problem concerning the amount of protons in cells, the question: "How many protons are there in a typical Escherichia coli cell? How many protons are in the periplasmic space?" I am struggling to calculate these numbers. I'm not sure if there is a specific formula I should be using or if I need to take into account the size/shape of the cell/periplasm. Any help would be greatly appreciated. Thanks!!!

John

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GreenDog
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Post by GreenDog » Sun Nov 13, 2005 2:38 pm

First of all, welcome to the forum! Hope we can help you.
Second, here is a site with some useful E.Coli data http://redpoll.pharmacy.ualberta.ca/CCDB/cgi-bin/STAT_NEW.cgi

Now, let's say that the internal pH of E.Coli is 7.5 and the cytoplasm volume is 6.7E-16 liter. This looks like all the information needed to calculate the amount of protons. I'm getting a stupid answer so if this helps to figure out the real answer I'd sure like to know.

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